Symptom severity and virus titer of transgenic N. benthamiana T1 plants, 30 days after challenge with BNYVV.

1− absence of symptoms, + leaf curling, ++ faint mosaic, mild stunting, +++ severe mosaic, leaf distortion, general stunting.2− value indicative of virus absence, + close to the positive threshold (three times the negative control), ++ half reading of positive control (non-transgenic), +++ equal to the positive control.3numbers denote number of plants in corresponding categories.4numbers in parentheses denote percent of plants in corresponding categories.*Data in the column refer to the averages from four independent experiments used as validation to the third column.