Expression changes in HeLa cells treated with siRNA against HCFC1 or control luciferase. Homo sapiens

We compared in triplicate mRNA levels from cells treated with siRNA against either HCF-1 or, as a negative control, luciferase. We observed that 19% of Refseq annotated genes are differentially expressed (either up or down regulated with a multiple testing corrected p value of ≤ 0.05) upon depletion of HCF-1. This large number of differentially expressed genes upon HCF-1 depletion demonstrates a broad role of HCF-1 in the regulation of gene expression. Overall design: The experiment includes biological triplicates of the expression changes between cycling HeLa cells treated with a control siRNA or siRNA against HCFC1.