Resveratrol and resveratrol-hydroxypropyl-β-cyclodextrin complex recovered the changes of creatine kinase and Na+, K+-ATPase activities found in the spleen from streptozotocin-induced diabetic rats

Abstract: Type 1 diabetes (T1D) is the result of the selective destruction of the pancreatic β-cells by T cells of the immune system. Although spleen is a secondary lymphoid organ, it is also involved in the T1D pathogenesis. However, the alterations in a variety of cellular processes of this disease need to be further understood. We aimed to analyze the benefits of resveratrol, and its complexed form on diabetic complications in the spleen of rats. To this end, we investigated important enzymes of phosphoryl transfer network, and Na+, K+-ATPase activity. Wistar rats were divided into non-diabetic groups: Control, Ethanol, Resveratrol, Hydroxypropyl-β-cyclodextrin, Resveratrol-hydroxypropyl-β-cyclodextrin, and diabetic groups with the same treatments. Diabetes was induced by a single dose of 60 mg/kg of streptozocin intraperitoneally, and treatments by intragastric gavage once daily for 60 days. Hyperglycemia reduced creatine kinase activity, which was reversed by the administration of resveratrol. Na+, K+-ATPase activity was greatly affected, but it was reversed by resveratrol and resveratrol-hydroxypropyl-β-cyclodextrin. This suggest an energetic imbalance in the spleen of diabetic rats, and in case this also occurs in the diabetic patients, it is possible that resveratrol supplementation could be beneficial to the better functioning of the spleen in diabetic patients.

摘要：1型糖尿病（Type 1 diabetes, T1D）是机体免疫系统T细胞选择性破坏胰腺β细胞所引发的疾病。脾脏作为次级淋巴器官，亦参与1型糖尿病的发病机制，但该疾病中多种细胞过程的异常改变仍有待进一步阐释。本研究旨在探究白藜芦醇及其复合制剂对大鼠脾脏糖尿病并发症的改善作用。为此，我们检测了磷酸转移网络中的关键酶活性以及Na+, K+-ATP酶（Na+, K+-ATPase）活性。将Wistar大鼠分为非糖尿病组与糖尿病组，两组均设置以下处理组别：对照组、乙醇组、白藜芦醇组、羟丙基-β-环糊精（Hydroxypropyl-β-cyclodextrin）组、白藜芦醇-羟丙基-β-环糊精组。糖尿病造模采用单次腹腔注射60 mg/kg链脲佐菌素（streptozocin），随后每日经胃管灌胃给药一次，持续60天。实验结果显示，高血糖会降低肌酸激酶（creatine kinase）活性，而白藜芦醇给药可逆转这一变化；Na+, K+-ATP酶活性受到显著影响，但白藜芦醇与白藜芦醇-羟丙基-β-环糊精均可使其恢复正常。上述结果表明，糖尿病大鼠脾脏存在能量失衡状态；若糖尿病患者体内也存在此类异常，那么补充白藜芦醇或可有助于改善糖尿病患者脾脏的正常功能。