Insertion sequence (IS)-content used in the species-specific qPCR assay.
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资源简介：
qPCR: quantitative polymerase chain reaction; B: Bordetella; Ct: cycle threshold; IS: insertion sequence.
aAn amount of 1 ng of DNA purified from stored samples was subjected to qPCR.
Serial dilutions of purified DNA from control isolates were used to evaluate the sensitivity of the different PCR reactions, which were approximately 7–8 bacteria/reaction.
bKindly provided by Pr. N. Guiso.
cHuman and animal isolates of B. bronchiseptica can sometimes contain few copies of IS481 and/or IS1001[22], [29].
创建时间：
2014-02-19
