Research data supporting "Co-transcriptional production of programmable RNA condensates and synthetic organelles"

Data repository for work contributing to the paper "Co-transcriptional production of programmable RNA condensates and synthetic organelles". The repository contains 24 ZIP archives and 1 README file describing the repository in detail. Epifluorescence timelapses, confocal micrographs and zoomed z-stacks data either reported in main text/SI or used for analysis are presented as pristine (no further processing) and displayed in TIFF format. Where single equatorial planes extracted from a z-stack were reported, entire pristine z-stacks are included. Depending on filesize, images might have been binned (specified in filename, e.g. "bin4x4"). For z-stacks data, images might have been binned (specified in filename, e.g. "bin2x2x2"). Gel images are included in a ZIP archive containing all (pristine and processed, i.e. inverted and/or rotation-corrected, gel images) reported in SI and used for analysis. Platereader fluorescence measurements were acquired using a BMG CLARIOstar Plus plate reader, data is presented in .csv format. See the main manuscript and SI (all open access) for further details on samples and data collection methods.

本数据集仓库服务于论文《共转录产生可编程RNA凝聚物与合成细胞器》的相关研究工作。该仓库包含24个ZIP归档文件与1份README文件，后者用于详细说明本仓库的整体情况。正文或补充材料中报道、或用于分析的落射荧光延时成像数据、共聚焦显微图像及放大Z轴堆叠数据集，均以未经过额外处理的原始（pristine）TIFF格式存储。若研究中使用了从Z轴堆叠中提取的单个赤道平面图像，则会同时提供完整的原始Z轴堆叠数据。根据文件体积大小，部分图像可能已进行分箱处理（文件名中会标注相关信息，例如"bin4x4"）。针对Z轴堆叠数据集，部分图像可能已进行三维分箱处理（文件名中会标注相关信息，例如"bin2x2x2"）。凝胶成像相关数据被打包至单个ZIP归档中，其中包含补充材料中报道及用于分析的全部原始与处理后（即经反色和/或旋转校正）的凝胶图像。酶标仪荧光检测数据由BMG CLARIOstar Plus酶标仪采集完成，数据以.csv格式存储。如需了解样本制备与数据采集方法的更多细节，请查阅该开源论文的正文及补充材料。