PacBio HiFi read metagenomes from water samples collected in the western Fram Strait using Remote Access Samplers

This project consists of the metagenome component of the larger FRAM RAS project. RAS stands for Remote Access Samplers, the autonomous sampling devices placed on moorings at specific locations in the Fram Strait region to collect samples year-round. In this project, water samples were collected during the 2016-2017 annual cycle and subsequently used for long-read, HiFi metagenome sequencing. At each sampling time point, ~1L of seawater was autonomously pumped into sterile plastic bags and fixed with mercuric chloride. After RAS recovery, water was filtered onto 0.22 µm Sterivex cartridges and samples frozen at -20°C until DNA extraction. Samples were sequenced at the Max Planck Genome Center, Cologne, using a PacBio Sequel II platform.