The primary transcriptome of Acinetobacter baumannii ATCC 17978

Transcriptomics by RNA-seq provides unparalleled insight into bacterial gene expression networks, enabling a deeper understanding of the regulation of pathogenicity, mechanisms of antimicrobial resistance, metabolism, and other cellular processes. Here we present the transcriptome architecture of Acinetobacter baumannii ATCC 17978, a species emerging as a leading cause of antimicrobial resistant nosocomial infections. Differential RNA-seq (dRNA-seq) examination of model strain ATCC 17978 in 16 laboratory conditions identified 3731 transcriptional start sites (TSS), and 110 small RNAs, including the first identification of 22 sRNA encoded at the 3′ end of mRNA. Transcriptome analysis of Acinetobacter baumannii ATCC 17978 from Early Stationary Phase (ESP) in L-broth and from a pool of 16 different environmental conditions using RNA-seq. Please note that one half of the RNA pooled sample [RNA_pool] was digested with Terminator Exonuclease (TEX) to enrich for 5'-PPP RNA species [i.e. TEX_RNA_pool].