Additional file 3: Figure S2. of The role of Tre6P and SnRK1 in maize early kernel development and events leading to stress-induced kernel abortion

Kernels harvested 3 DAP were tested for long term viability in culture. After sterilization, kernels were plated on MS agar and incubated in the dark at 24 °C. Control kernels on continuous sucrose (150 mM) are indicated with closed (black) squares and sucrose starved kernels are indicated by white (open) squares. Means are plotted using 4 biological replicates consisting of 3 plants each. (XLSX 24 kb)

于授粉后3天（days after pollination，DAP）收获的籽粒，于离体培养条件下开展长期活力检测。经灭菌处理后，将籽粒接种于MS琼脂培养基（MS agar），并置于24℃黑暗环境中培养。持续添加150 mM蔗糖的对照组籽粒以实心（黑色）方块标记，蔗糖饥饿处理组籽粒以空心（白色）方块标记。本次均值统计采用4次生物学重复，每个重复包含3株植株。（XLSX格式，24 KB）