Integrated genomic and transcriptomic analysis improves disease classification and risk stratification of MDS with ring sideroblasts

Full transcriptome (RNA-sequencing) from bulk CD34+ bone marrow mononuclear cells from MDS patients with ring sideroblasts. CD34+ cells were isolated from the MNC using AUTO-MACS with double-separation option (Miltenyi Biotec, Germany) and submitted for RNA extraction. RNA was extracted with RNeasy Microkit (Qiagen, Hilden, Germany) and treated with DNase, according to manufacturer instruction. RNA integrity number was estimated using Agilent RNA 6000 Pico (Agilent Technologies, Palo Alto, CA) and was greater than 6.5 for all the samples (median 8.2). The RNA-sequencing (RNA-seq) libraries were prepared from total RNA using SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian with enzymatic ribosomal depletion (Takara Bio, Japan). Libraries were sequenced using the Novaseq 6000 with paired-end 150bp configuration. The molecular data were integrated with clinical information aiming to improve prognosis prediction in this hematologic malignancy. The dataset consists of 2 files: - FASTQ_RS.tar.gz: compressed folder that includes 258 fastq files - metadata_RS.xlsx The total size of the dataset is approximately 1 TB.伴环形铁粒幼红细胞的骨髓增生异常综合征（Myelodysplastic Syndromes, MDS）患者的批量CD34阳性骨髓单个核细胞的全转录组（RNA测序，RNA-seq）数据。研究人员采用德国美天旎生物科技（Miltenyi Biotec）公司的AUTO-MACS双分选模式，从骨髓单个核细胞（mononuclear cells, MNC）中分离CD34阳性细胞，用于后续RNA提取实验。依照试剂盒说明书，使用德国希尔德市凯杰（Qiagen）公司的RNeasy微量试剂盒提取总RNA，并以DNase进行消化处理。采用美国安捷伦科技（Agilent Technologies）公司的Agilent RNA 6000 Pico试剂盒检测所有样本的RNA完整性数值（RNA Integrity Number, RIN），所有样本的RIN值均大于6.5，中位数为8.2。使用日本宝生物（Takara Bio）公司的SMARTer链特异性总RNA测序试剂盒v2 微量哺乳动物样本版（搭配酶法核糖体RNA去除步骤），从总RNA中构建RNA测序（RNA-seq）文库。采用Novaseq 6000测序平台，以双端150bp的测序模式完成文库测序。本研究将分子数据与临床信息整合，以期优化该血液系统恶性肿瘤的预后预测效能。 本数据集包含2个文件： - FASTQ_RS.tar.gz：压缩文件夹，内含258个FASTQ格式测序文件 - metadata_RS.xlsx：元数据表格文件 数据集总容量约为1 TB。