Effects of DMSO on the morphology and physiology of zebrafish embryos. DMSO can significantly, in a dose-dependent manner, alter the morphology and physiology of zebrafish embryos, including changes in embryo’s size and curvature, heart beating frequency, pericardium area, somite size and myofibril alignment, melanocyte size, and morphology of notochord and swim bladder. (*) only the swim bladder area was quantified in 7 dpf zebrafish larvae treated with 2% DMSO, while all other measurements were quantified in 72 hpf zebrafish embryos treated with 4% DMSO. CI = curvature index.

Effects of DMSO on the morphology and physiology of zebrafish embryos. DMSO can significantly, in a dose-dependent manner, alter the morphology and physiology of zebrafish embryos, including changes in embryo’s size and curvature, heart beating frequency, pericardium area, somite size and myofibril alignment, melanocyte size, and morphology of notochord and swim bladder. (*) only the swim bladder area was quantified in 7 dpf zebrafish larvae treated with 2% DMSO, while all other measurements were quantified in 72 hpf zebrafish embryos treated with 4% DMSO. CI = curvature index.