The impact of IL-33 on Srebf2-dependent cholesterol gene expression in ST2+ eVAT Tregs

Tregs are highly enriched in the eVAT of male mice. We show that ST2+ VAT Tregs preferentially upregulate and depend on Srebf2-mediated cholesterol metabolism compared their spleen Treg or ST2- VAT Treg counterparts. However, it is unclear whether this increased reliance on cholesterol metabolism is a consequence elevated IL-33 signaling in ST2+ Tregs. Therefore, the goal of experiment is to determine whether IL-33 signaling influences Srebf2-dependent cholesterol gene expression in ST2+ eVAT Tregs in vivo. Foxp3-YFPcre Srebf2 wt/wt or Srebf2 flox/flox mice were given 2 doses of either PBS or 2ug recombinant IL-33 (3 days apart) the final week before sacrifice. 1000 ST2+ Tregs were isolated and sort-purified for bulk-RNA sequencing.