Future H1N1 and H3N2 N-linked glycosylation sequon locations predicted by FSF. Each table summarizes the top site predictions from 10 individual FSF runs using currently circulating IFV HAs. Overall near-sequon rankings were determined by site-specific mutation rates, with higher rates corresponding to higher overall rankings. Near-sequon frequencies among the analyzed strains are displayed as proportions, alongside the average side-chain exposure ratios of the first amino acids (AAs) in the near-sequon triads. The mutation rates for the mismatched AA positions and their gamma category assignments are also presented, along with the near-sequon distinction of the site and its ranking among other near-sequons based on mutation rates. (Top) Future H1N1 sequon locations predicted by FSF using a mutation frequency calculation based on a random selection of H1N1 HA sequences per year for all years from 1918-2024. (Middle) Future H1N1 sequon locations predicted by FSF using a mutation fr

Future H1N1 and H3N2 N-linked glycosylation sequon locations predicted by FSF. Each table summarizes the top site predictions from 10 individual FSF runs using currently circulating IFV HAs. Overall near-sequon rankings were determined by site-specific mutation rates, with higher rates corresponding to higher overall rankings. Near-sequon frequencies among the analyzed strains are displayed as proportions, alongside the average side-chain exposure ratios of the first amino acids (AAs) in the near-sequon triads. The mutation rates for the mismatched AA positions and their gamma category assignments are also presented, along with the near-sequon distinction of the site and its ranking among other near-sequons based on mutation rates. (Top) Future H1N1 sequon locations predicted by FSF using a mutation frequency calculation based on a random selection of H1N1 HA sequences per year for all years from 1918-2024. (Middle) Future H1N1 sequon locations predicted by FSF using a mutation fr