Exoproteomics data for "Dissolved Organic Phosphorus Bond-Class Utilization by <i>Synechococcus</i>"

This data is part of the submitted manuscript “Dissolved Organic Phosphorus Bond-Class Utilization by <i>Synechococcus</i>” by Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, and Solange Duhamel. This dataset aims to identify the enzymes expressed when picophytoplankton <i>Synechococcus</i> is phosphorus stressed. <i>Synechococcus </i>strains WH8102 (open-ocean strain isolated from the Atlantic Ocean) and WH5701 (coastal strain isolated from Maine, US) were grown in the Duhamel Lab at the University of Arizona. Axenic culture was grown in triplicate 500 mL culture flasks in SN media amended with 1 µmol L^-1 KH₂PO₄; a concentration previously determined as being low enough to induce phosphorus stress while still maintaining biomass (Cox and Saito, 2013). Peptide samples were analyzed at the Proteomics and Mass Spectrometry (PAMS) facility at the University of Georgia on a Thermo-Fisher LTQ Orbitrap Elite mass spectrometer coupled with a Proxeon Easy NanoLC system (Waltham, MA, United States) following Adams <i>et al.</i> (2022). Peptides were mapped to the <i>Synechococcus</i> genome (NCBI BioProject PRJNA230) (Palenik <i>et al.</i> 2003; McCarren <i>et al.</i> 2005). The protein set was searched in BLASTP against NCBI non-redundant databases, and accession numbers were cross-referenced on UniProt to confirm putative function and identify alkaline phosphatases.<br><i>For inquiries regarding the contents of this dataset, please contact the Corresponding Author listed in the README.txt file. Administrative inquiries (e.g., removal requests, trouble downloading, etc.) can be directed to [e-mail]</i><br>

本数据集隶属于已投稿的学术手稿《聚球藻（*Synechococcus*）对溶解有机磷键类的利用》，作者为Emily M Waggoner、Kahina Djaoudi、Julia M Diaz与Solange Duhamel。本数据集旨在探究超微型浮游植物聚球藻（*Synechococcus*）在磷胁迫条件下所表达的酶类。
实验所用聚球藻菌株包括WH8102（分离自大西洋的远洋株系）与WH5701（分离自美国缅因州的沿岸株系），均于亚利桑那大学杜哈梅尔实验室完成培养。无菌培养（Axenic culture）采用500 mL培养瓶进行三次生物学重复，培养基为添加了1 μmol·L⁻¹磷酸二氢钾（KH₂PO₄）的SN培养基；该浓度经前期研究证实可诱导磷胁迫，同时足以维持生物量（Cox与Saito, 2013）。
肽样品的检测于佐治亚大学蛋白质组学与质谱（Proteomics and Mass Spectrometry, PAMS）平台完成，使用赛默飞世尔（Thermo-Fisher）LTQ Orbitrap Elite质谱仪，联用Proxeon Easy NanoLC系统（美国马萨诸塞州沃尔瑟姆），实验流程参照Adams等人（2022）的方法。肽段序列被比对至聚球藻基因组（美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）生物项目编号PRJNA230；Palenik等人, 2003; McCarren等人, 2005）。
蛋白质组通过BLASTP比对至NCBI非冗余数据库，并在通用蛋白质知识库（Universal Protein Resource, UniProt）中交叉引用登录号以确认推定功能，同时鉴定碱性磷酸酶。
若对本数据集的内容存在疑问，请联系README.txt文件中列出的通讯作者。行政类咨询（如删除请求、下载故障等）可发送至邮箱[e-mail]。

搜集汇总

背景与挑战

背景概述

该数据集是一个外蛋白质组学数据集，旨在研究聚球藻（Synechococcus）在磷胁迫下对溶解有机磷键类的利用机制。通过分析两种聚球藻菌株在低磷条件下的肽段表达，识别了相关酶（如碱性磷酸酶），并映射到基因组以确认功能，为海洋磷循环研究提供关键实验数据。