single cell RNA sequencing of SMC specific Yap1 knockout mouse aorta

When aortic cells are under stress, such as increased hemodynamic pressure, they adapt to the environment by modifying their functions, allowing the aorta to maintain its strength. To understand the regulation of this adaptive response, we examined the transcriptomic and epigenomic programs in aortic smooth muscle cells (SMCs) during the adaptive response to angiotensin II (AngII) infusion and determined its importance in protecting against aortic aneurysm and dissection. Overall design: To understand to adaptive role of Yap1 in smooth muscle cells (SMCs) of aorta, we performed single cell RNAseq of aortic tissues from wild type mice, AngII infused wild type mice, SMC-specific Yap1 KO mice, AngII infused SMC-specific Yap1 KO mice.