Identification of candidate immunity biomarkers associated with age-related variations in osteoclast activity following orthodontic tooth movement

This study provides a comprehensive examination of the influence of age on osteoclast activity during orthodontic tooth movement, analyzed through the lens of osteoimmunology, identifying key immune molecules and cells involved in the regulation.We identified key molecules that play an important role in orthodontic bone reconstruction by analyzing the differences in immune inflammatory differential gene expression across different age groups. These molecules, which facilitate macrophage migration to the local site and their differentiation into osteoclasts, as well as directly enhance osteoclast differentiation and function, are differentially expressed in young groups, but not in adult groups. Overall design: Gene expression profiling analysis of RNA-seq data from C57BL/6 mice of two different age groups, with each group subjected to an orthodontic tooth movement (OTM) model established between the maxillary first molar and maxillary incisors on the left side for durations of 7 and 14 days.