Study on the effect of the treatment of Periplaneta americana L. extract Ento-B by Dinitrochlorobenzene combined with acetic acid induced UC in rats

ABSTRACT Purpose To study the Periplaneta americana L. extract Ento-B on the treatment of chronic ulcerative colitis induced by 2,4-dinitrochlorobenzene and acetic acid in rats and to explore its primary mechanism of action. Methods Using 2,4-dinitrochlorobenzene combined with acetic acid to induce chronic ulcerative colitis (chronic UC) in rats. The sulfasalazine (400 mg/kg) and Ento-B (200 mg/kg, 100 mg/kg,50 mg/kg) were given by intragastric administration and the effect was evaluated according to the disease activity index (DAI) score, colon mucosal injury index (CMDI) score, histopathological score (HS) and the serum levels of Interleukin-4(IL-4), Interleukin-10(IL-10), Tumor necrosis factor-α(TNF-α), Malondialdehyde(MDA), Superoxide dismutase(SOD) and Inducible nitric oxide synthase(iNOS.) Results Compared with the model group, all doses of Ento-B could reduce the score of CMDI (p < 0.05), HS(p < 0.05 or p < 0.01), significantly increased the expression of IL-4, IL-10, SOD (p < 0.01) and decreased the levels of TNF-α, MDA, iNOS in serum of UC rats, significantly improving the degree of colon lesionsin UC rats. Conclusions Ento-B may play an important role in the treatment of ulcerative colitis induced byUC rats. The mechanism may be related to the increased expression of IL-4, IL-10, SOD and reduced expression of TNF-α, MDA, iNOS.

摘要 研究目的 探究美洲大蠊（Periplaneta americana L.）提取物Ento-B对2,4-二硝基氯苯联合乙酸诱导的大鼠慢性溃疡性结肠炎的治疗作用，并初步探讨其作用机制。实验方法 采用2,4-二硝基氯苯联合乙酸构建大鼠慢性溃疡性结肠炎（chronic UC）模型，通过灌胃给药给予柳氮磺吡啶（sulfasalazine，400 mg/kg）以及三种剂量的Ento-B（200 mg/kg、100 mg/kg、50 mg/kg）；以疾病活动指数（DAI）评分、结肠黏膜损伤指数（CMDI）评分、组织病理学评分（HS），以及血清中白细胞介素-4（IL-4）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）、超氧化物歧化酶（SOD）和诱导型一氧化氮合酶（iNOS）水平作为评价指标，评估各组的治疗效果。实验结果 与模型组相比，各剂量的Ento-B均可降低结肠黏膜损伤指数（CMDI）评分（p<0.05）与组织病理学评分（HS）（p<0.05或p<0.01）；可显著升高溃疡性结肠炎大鼠血清中IL-4、IL-10及SOD的表达水平（p<0.01），同时降低血清中TNF-α、MDA及iNOS的水平，有效改善溃疡性结肠炎大鼠的结肠病变程度。研究结论 Ento-B对溃疡性结肠炎模型大鼠具有明确的治疗作用，其作用机制可能与上调IL-4、IL-10及SOD的表达，下调TNF-α、MDA及iNOS的表达相关。