Data from: Rapid and accurate detection of urinary pathogens by mobile IMS-based electronic nose: a proof-of-principle study

Urinary tract infection (UTI) is a common disease with significant
morbidity and economic burden, accounting for a significant part of the
workload in clinical microbiology laboratories. Current clinical chemisty
point-of-care diagnostics rely on imperfect dipstick analysis which only
provides indirect and insensitive evidence of urinary bacterial pathogens.
An electronic nose (eNose) is a handheld device mimicking mammalian
olfaction that potentially offers affordable and rapid analysis of samples
without preparation at athmospheric pressure. In this study we demonstrate
the applicability of ion mobility spectrometry (IMS) –based eNose to
discriminate the most common UTI pathogens from gaseous headspace of
culture plates rapidly and without sample preparation. We gathered a total
of 101 culture samples containing four most common UTI bacteries: E. coli,
S. saprophyticus, E. faecalis, Klebsiella spp and sterile culture plates.
The samples were analyzed using ChemPro 100i device, consisting of IMS
cell and six semiconductor sensors. Data analysis was conducted by linear
discriminant analysis (LDA) and logistic regression (LR). The results were
validated by leave-one-out and 5-fold cross validation analysis. In
discrimination of sterile and bacterial samples sensitivity of 95% and
specificity of 97% were achieved. The bacterial species were identified
with sensitivity of 95% and specificity of 96% using eNose as compared to
urine bacterial cultures. In conclusion: These findings strongly
demonstrate the ability of our eNose to discriminate bacterial cultures
and provides a proof of principle to use this method in urinanalysis of
UTI.