Transcriptome sequencing analysis for NCI-H209 lung tumor cells conditioned by human brain microvascular endothelial cells

Endothelial cells of blood vessel playing a key role in the metastasis of tumor cells particularly the brain microvascular endothelial cells in the brain-tropic metastasis of lung tumor cells. Currently, studies towards the influence contributed by tumor cells to the alteration of endothelial cells have been extensively conducted by contrast studies for the alteration of gene-expression signature of tumor cells under the influence of endothelial cells remains poorly characterized, thus in this study the transcriptome of the human small-cell lung cancer cell NCI-H209 was sequenced via RNA-Seq after conditioned by human brain microvascular endothelial cell (HBMEC) for characterizing the alteration of gene-expression signature. mRNA expression signatures of 5 samples of tumor cells were profiled, among of which three samples belonging to control group and the other two making up the experiment group conditioned by human brain microvascular endothelial cells