智能纳米药物的构建及靶向治疗脑部疾病

脑部疾病精准靶向治疗具有广阔的应用前景，尤其是采用具有多功能、靶向性、智能化的纳米粒子作为药物载体，能够实现脑胶质瘤、阿尔茨海默症等脑部相关疾病的高效治疗。“智能纳米药物的构建及靶向治疗脑部疾病”采用智能纳米递送系统进行药物递送，大幅度提高了药物利用度、改变药物组织器官分布、提高药物生物安全性。该研究面向国家“脑科学与类脑研究”重大科技项目研究方向，服务社会医疗健康。本数据集涉及多种纳米药物（核酸类纳米药物、仿生纳米药物、基因药物等）的制备以及其结构表征，纳米药物的药学、药效学、毒理评价。基于核磁以及质谱表征纳米载体的化学结构，基于傅里叶红外光谱仪表征纳米载体的化学结构，基于纳米粒度及zeta电位分析仪和透射电子显微镜表征纳米粒子的粒径分布以及形貌，基于紫外可见吸收研究纳米粒子在体外释放情况，基于流式细胞仪验证纳米粒子被细胞内吞的表征，基于酶标仪对药物体外释放的表征，基于小动物活体成像系统探究纳米粒子的活体脑靶向，基于激光共聚焦显微镜观察切片验证在脑内富集情况，基于包埋机包埋和切片，基于玻片扫描显微成像系统扫描免疫组化的切片。数据量22.3G。

Precision targeted therapy for brain diseases has broad application prospects. In particular, the use of multifunctional, targeted and intelligent nanoparticles as drug carriers enables efficient treatment of brain-related diseases such as brain glioma and Alzheimer's disease. The project titled "Construction of Intelligent Nanomedicines and Targeted Therapy for Brain Diseases" utilizes intelligent nanoscale drug delivery systems for drug delivery, which significantly improves drug bioavailability, modulates the tissue and organ distribution of drugs, and enhances drug biosafety. This research aligns with the research direction of the national major science and technology project "Brain Science and Brain-Inspired Research" and serves social medical and health causes. This dataset covers the preparation and structural characterization of various nanomedicines (including nucleic acid-based nanomedicines, biomimetic nanomedicines, gene drugs, etc.), as well as the pharmaceutical, pharmacodynamic and toxicological evaluations of nanomedicines. The chemical structures of nanocarriers are characterized via Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS), as well as Fourier Transform Infrared Spectroscopy (FTIR). The particle size distribution and morphology of nanoparticles are characterized using a nanoparticle size and zeta potential analyzer and Transmission Electron Microscopy (TEM). The in vitro release profile of nanoparticles is investigated via Ultraviolet-Visible (UV-Vis) absorption spectroscopy. The cellular internalization of nanoparticles is validated via flow cytometry, and the in vitro drug release is characterized using a microplate reader. The in vivo brain targeting performance of nanoparticles is evaluated using a small animal in vivo imaging system. The enrichment of nanoparticles in the brain is confirmed by examining tissue sections under a laser confocal scanning microscope. Tissue embedding and sectioning are conducted using an embedding machine, and immunohistochemical (IHC) slides are scanned with a slide scanning microscopy system. The total size of this dataset is 22.3 GB.