RNA-Seq on Primary Mouse Hepatocytes Control condition
收藏Zenodo2024-01-12 更新2026-05-26 收录
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https://zenodo.org/doi/10.5281/zenodo.10495829
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After extractions, cells were cultured in 5% CO2 at 37°C using Williams E media supplemented with 2.24 g/l NaHCO3, 100 U/100 µM penicillin/streptomycin, 100 µM dexamethasone and 2 mM L-glutamine.
RNA was isolated using the RNeasy® Mini Kit (Qiagen, 74104) on-column DNA digestion was performed.
Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs GmbH, Germany)
Cells were treted with DMSO during 48h.
Genome assembly: mm10
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Zenodo创建时间：
2024-01-12
