Microbial genome collection of aerobic granular sludge cultivated in sequential batch reactor using different carbon source mixtures

Abstract Aerobic granular sludge microogranisms are cultivated in a sequential batch reactor (SRB), and samples were fed different carbon source mixtures, including volatile fatty acids (day 71), complex monomeric (day 322 and 427), and complex polymeric (day 740). This study utilizes the dataset PRJEB38840 (Adler et al 2022 Sfam) to construct a hybrid assembly of Illumina short reads (two samples: DNA extraction A and B) and PacBio long reads (one sample: DNA extraction A) using the SPAdes assembler. The resulting metagenomic assemblies are presented for each of the days 71, 322, 427, 740. From this work, 478 new quality-controlled MAGs were obtained. Combined with 275 unpublished MAGs (Adler et al) and 6 MAGs from Aline adler, Sfam 2022. Making a in-house genome collection of 759 MAGs in total. These MAGs have been dereplicated to obtain representatives (n=233). The whole MAG collection is indexed using the gtdb taxonomy and are accompanied by key metrics such as genome size, number of contigs, and N50 value. Additionally, the study offers R-codes for data analysis.