Relative luciferase activity 24 h after the transfection of various cell lines with constructs with lesions at codons 344 and 422.

aNTS: nontranscribed strand; TS: transcribed strand ; G*: 8OG.
bValues are expressed as [(RLUPp/RLURr)construct/(RLUPp/RLURr)100%]×100 in the same cell line. Each value is the mean of at least six replicate samples. RLU: relative light units.
cp values for Mann-Whitney U test are 0.0022 for comparisons with the relative luciferase activities of MEF cells transfected with the same construct.
dDistributions were considered to be significantly different when p<0.05.
eThe dose of doxycycline was reduced from 2 µg/ml to 1 ng/ml.