Additional file 6: Figure S4. of Whole-genome bisulfite sequencing of cell-free DNA identifies signature associated with metastatic breast cancer

Integrated Genomics Viewer screenshots of WGBS and MiSeq sequencing results shown by gene for GP5 (A), HTR1B (B), PCDH10 (C), and UNC13A (D). The top panel, separated by a thick black line, shows a histogram representing the percent methylation value for each CpG locus in each amplicon for H, DFS, and MBC. In the lower panel, histograms display the percent unmethylated reads (blue) and percent methylated reads (red) at each assayed CpG locus. Below each of these histograms are the individual reads. (ZIP 634 kb)

针对GP5（A）、HTR1B（B）、PCDH10（C）与UNC13A（D）四个基因，本数据集提供了其全基因组亚硫酸氢盐测序（Whole Genome Bisulfite Sequencing，WGBS）及MiSeq测序结果的整合基因组浏览器（Integrated Genomics Viewer）截图。
图中上方区域以粗黑线分隔，展示了H、DFS、MBC三组样本中，每个扩增子（amplicon）内各CpG位点的甲基化百分比直方图。
下方区域的直方图则分别呈现了各检测CpG位点的未甲基化读取序列占比（蓝色）与甲基化读取序列占比（红色）。
各直方图下方均附有单条测序读段的可视化结果。
（ZIP 634 kb）