Xrp1 governs the stress response program to spliceosome dysfunction

We investigated the changes in gene expression in response to reduced levels of Ecdysoneless (Ecd) in the developing wing disc of Drosophila. To do this we utilized the GAL4/UAS system to express UAS-ecd-RNAi in the nubbin-domain (nub-Gal4, UAS-mRFP, UAS-ecd-RNAi) of the wing disc and used Nub-Gal4/+ wing discs as controls.  We quantified transcriptomic expression analysis from four biological replicates for both experimental groups mRNA profiles were generated by single-end, total RNA Illumina sequencing (HiSeq2500) from wandering L3 stage wing discs of nub-Gal4, UAS-mRFP, UAS-ecd-RNAi and nub-Gal4, UAS-mRFP Drosophila larvae.