16S rRNA gene amplicon sequencing of fecal microbiota from Salmonella Typhimurium-infected mice treated with rutin

This project performed 16S rRNA gene amplicon sequencing targeting the V4 region to characterize gut microbiota composition in a murine Salmonella Typhimurium infection model. Female BALB/c mice were divided into control, infected, and rutin-treated groups. Fecal samples were collected and subjected to Illumina-based 16S rRNA sequencing. The aim of this study is to evaluate infection-induced microbial dysbiosis and the modulatory effects of rutin treatment on gut microbial community structure.