Temperature and Humidity Dependent progression and maturation rate of mosquito

The study involved rearing mosquitoes in simulated temperatures and relative humidity. A total of four hundred (400) eggs of An. arabiensis were obtained from JU TIDRC insectary and reared to adults. Mosquitoes were reared across temperatures ranging from the minimum 14.55°C to maximum 34.35°C and relative humidity spanning from 64% to 86%. Each temperature and humidity combination were replicated four times, with one hundred mosquito eggs placed in petri dishes containing wet filter paper. After a 24-hour period, the eggs were carefully washed and transferred into hatching trays. Throughout the larval stage, adequate nourishment was provided, and observations were recorded until pupation occurred.
Subsequently, pupae were relocated into cages, and the emergence of adult mosquitoes was closely monitored until the end of their lifespan. Detailed records were maintained, capturing key parameters such as the number of days required for egg hatching, as well as the survival or mortality rates of eggs hatching, larvae, pupae and adult mosquitoes under each temperature and humidity condition.