Expression data from regulatory T cells in mice

It is well accepted that the generation and functional stability of Treg cells stand as one dominant force to maintain immunological homeostasis during steady-state or under inflammatory conditions, as the primary Treg cell deficiency causes numerous types of autoimmunopathy, hypersensitivity or inflammation-induced carcinogenesis. We used microarrays to indentify the global programme of gene expression underlying regulatory mechanism and explore the differentially expressed genes during the process of immunology regulation. WT or KO naïve T cells were freshly isolated from spleen and stimulated under Treg-induction conditions for 3 days. Cells were collected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain differential genes between WT and KO Treg cells.