Video recording of freely diffusing 200nm polystyrene nanoparticles using dark field microscopy

Dark field recording of 200nm polystyrene (Kisker Biotech PPs-02) particles which were diluted in essentially particle-free double distilled H2O. The temperature was 25 °C. The concentration was amounted to approximately 3x10^9 ml. The video file was captured with a PCO Edge CCD camera at 30 FPS mounted on a Olympus BX51 microscope (100-fold oil immersion objective) which was illuminated with a CytoViva dark field oil condenser. The pixel size of the video is 63nm. Please note that the video is splitted into mutiple archives. Please download all archives to get the full video. <strong>This video is part of a puplication:</strong> <strong>Wagner, T., Lipinski, H.-G. &amp; Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x</strong>

本数据集为200nm聚苯乙烯（polystyrene，Kisker Biotech PPs-02）颗粒的暗场（dark field）成像记录，实验所用颗粒以基本不含颗粒的双蒸水（double distilled H₂O）进行稀释。实验温度为25 ℃，颗粒浓度约为3×10⁹ 个/毫升。视频文件由搭载于奥林巴斯（Olympus）BX51显微镜的PCO Edge CCD（Charge-Coupled Device）相机拍摄，拍摄帧率为30 FPS，该显微镜配备100倍油浸物镜，并通过CytoViva暗场油聚光镜进行照明。
视频的像素尺寸为63 nm。请注意，该视频已被拆分为多个分卷存档，请下载所有分卷以获取完整视频。
<strong>本视频属于一篇已发表学术论文的配套数据：</strong>
<strong>Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. 暗场纳米颗粒追踪分析（nanoparticle tracking analysis）用于等离激元与非等离激元颗粒的尺寸表征. 《纳米颗粒研究杂志（Journal of Nanoparticle Research）》，16(5)，第2419页. 可访问：http://link.springer.com/10.1007/s11051-014-2419-x</strong>