scRNA-sequencing of mesenchymal cells and immune cells sorted from the lung of PBS or HDM challenged mice

We compared the transcriptomes of mesenchymal cells and immune cells in mice sensitized and challenged with house dust mite (HDM) extracts and those in control PBS-treated mice, using single cell RNA-seq (scRNA-seq). Data with mesenchymal cells and data with immune cells were obtained from separate experiments. For scRNA-seq of mesenchymal cells, each sample was pooled from cells from 3 mice and 2 different samples per group (for a total of 6 mice) were used. For scRNA-seq of immune cells, each sample was from cells of one individual mouse. Overall design: We used the 10X Genomics platform to prepare scRNA-seq libraries to detail the global programme of different gene expression in mesenchymal cells and immune cells in the lungs between PBS and HDM challanged mice .