Oryza sativa Transcriptome or Gene expression. Oryza sativa

OsPI1 was isolated from rice and revealed to belong to the TPSI1/Mt4 family, a phosphorus deficiency responsive gene family. The importance of this family gene for phosphorus deficiency responses of plants had been already reported, though its actual role is still unclear. To understand the plants response mechanisms for phosphorus deficiency and the role of OsPI1 in it, we constructed a OsPI1 RNAi line (ospi1-2) for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the shoots and roots of ospi1-2 and Oryza sativa L. cv. Kitaake wild type (WT) rice plants under constitutive and phosphorus deficiency at seedling stage. Keywords: Influences of OsPI1 knockdown Overall design: We used Agilent rice gene chips (G4138A) to investigate the transcript level changes in rice plant tissues during phosphorus deficiency. We used an OsPI1 knockdown line (ospi1-2) and wild type (WT). Plants were grown in growth chambers and treated in nutrient solutions with or without 32 μM NaH2PO4 for 9 d. Three plants were harvested for one replication and each sample was divided into roots and shoots. Two replications of microarray experiments using two biological replications were carried out by hybridizing the RNA from ospi1-2 and WT plants on the same slide.