comparison of mRNA expression among rat hepatoma cell lines with different metastatic ability

To reduce cancer mortality, understanding of mechanisms of cancer metastasis is crucial. We have established 6 rat hepatocellular carcinoma (HCC) cell lines, which exhibit differing metastatic potential to the lung after inoculation into the tail veins of nude mice. Micorarray analysis of 4 kinds of HCC cells and rat normal liver tissue was performed to find a potent molecular target for prevention of cancer metastasis. By the microarray analysis, mRNA expression was compared among two low-metastatic rat HCC cell lines (C5F and C6) and two high-metastatic rat HCC cell lines (N1 and L2) as well as non-treated rat liver tissue (as a reference sample).