The Impact of Biosynthesized ZnO Nanoparticles from Olea europaea (Common Olive) on Pseudomonas Aeruginosa Growth and Biofilm Formation. The Impact of Biosynthesized ZnO Nanoparticles from Olea europaea (Common Olive) on Pseudomonas Aeruginosa Growth and Biofilm Formation

the impact of different concentration of ZnO NPs on the relative gene expression of QS-regulatory genes Overall design: qPCR gene expression profiling.qRT-PCR was used, the total bacterial RNA was extracted at the middle of the log phase, corresponding to OD600 of 0.5-0.6, from PA subjected to a fairly low concentration (300 µg/ml) and high level (900 µg/ml) of ZnO NPs dispersed in the the MH mediumpooled prior to gene expression analysis.