Cleaving DNA with DNA
收藏PubMed Central1998-03-03 更新2026-04-25 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC19303/
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资源简介：
A DNA structure is described that can cleave single-stranded DNA oligonucleotides in the presence of ionic copper. This “deoxyribozyme” can self-cleave or can operate as a bimolecular complex that simultaneously makes use of duplex and triplex interactions to bind and cleave separate DNA substrates. Bimolecular deoxyribozyme-mediated strand scission proceeds with a k(obs) of 0.2 min(−1), whereas the corresponding uncatalyzed reaction could not be detected. The duplex and triplex recognition domains can be altered, making possible the targeted cleavage of single-stranded DNAs with different nucleotide sequences. Several small synthetic DNAs were made to function as simple “restriction enzymes” for the site-specific cleavage of single-stranded DNA.
提供机构：
National Academy of Sciences
创建时间：
1998-03-03
