Impact of microbiome depletion and SFB on oropharyngeal candidiasis

The goals of this study were to determine the impact of segmented filamentous bacteria on oral immunity to the commensal fungus Candida albicans in a mouse model of oropharyngeal candidiasis (OPC). SFB is a constituent of the mouse intestinal microbiome, but can exert extra-intestinal impacts on 'Type 17' cell immunity. Gnotobiotic C57BL/6 mice were gavaged with SFB and after 14 days infected orally with C. albicans (strain CAF2-1). Two days later, whole RNA from tongue was isolated and subjected to RNASeq.