Uridylation is a genomic feature of encapsidated GFLV RNAs [GFLV]

To determine whether the single 3' terminal uridine of the positive strand is encoded and whether this feature is shared by the negative strand, we attempted to map the 5' and 3' ends of the minus strand for both GFLV RNA1 and RNA2. Overall design: Using 3' and 5' RACE-seq method, we sequenced 3' and 5' extremities of plus and minus strands of GFLV RNAs (GT isolate).