Carbapenem Resistance in Clinical Isolates of Escherichia Coli among Patients Visiting A Tertiary Care Hospital, in Central India

Introduction: The emergence and increase of Carbapenem resistance in Escherichia coli is now posing a serious threat to human health around the world. Our study aimed to investigate the phenotypic and genotypic detection of Carbapenem resistance among E. coli isolates. Material Method: The present study was carried out in the Department of Microbiology, Index Medical College, Hospital & Research Centre (IMCHRC) Indore (M.P.). Various clinical samples were collected from the patients attending Medical College, Hospital & Research Centre (IMCHRC) Indore (M.P.). Total 215 E.coli isolates were examined regarding age, sex, departments and Carbapenemase resistance among different clinical specimens such as urine, pus, blood, CSF and respiratory secretions received in microbiology laboratory. Results: It is observed that out of 215 E.coli isolates, about 153 (71.16%) isolates were from male patients while 62 (28.83%) were from female patients. The majority (53.95%) of isolates were from urine samples. Out of 215 E. coli isolates, total 63 (29.30%) were carbapenem resistant. Conclusion: the rate of Carbepenem resistant E.coli is rising very quickly and becoming most important problem in the part of infectious diseases. Hence, now it is very important to detect the changing pattern of resistance as soon as possible to prevent the sread of resistant bacteria and modifying the strategies of treatment.      研究背景：耐碳青霉烯类大肠埃希菌（Escherichia coli）的出现与流行目前正对全球人类健康造成严重威胁。本研究旨在探究大肠埃希菌分离株中碳青霉烯类耐药性的表型与基因型检测方案。 材料与方法：本研究于印度中央邦印多尔市指数医学院附属医院与研究中心（Index Medical College, Hospital & Research Centre, 简称IMCHRC）微生物学科开展。研究样本采集自就诊于该医疗机构的患者，共计纳入215株大肠埃希菌分离株，针对患者年龄、性别、收治科室，以及尿液、脓液、血液、脑脊液、呼吸道分泌物等不同临床标本中的碳青霉烯酶耐药性情况进行分析，所有标本均送检于该中心微生物实验室。 研究结果：在215株大肠埃希菌分离株中，153株（71.16%）来源于男性患者，62株（28.83%）来源于女性患者。大部分分离株（53.95%）来源于尿液标本。215株分离株中共计63株（29.30%）表现为碳青霉烯类耐药。 研究结论：耐碳青霉烯类大肠埃希菌的流行速率正快速攀升，已成为感染性疾病防控领域的重大挑战。因此，尽快明确耐药性的变化模式以阻断耐药菌的传播、优化临床治疗策略，当前已显得尤为关键。