Biomarker responses (serum biochemistry) in pregnant female wistar rats and histopathology of their neonates exposed prenatally to pesticides

Experiments were conducted to investigate the effects on health of pregnant female rats exposed to pesticides glyphosate and carbendazim. Glyphosate is used as herbicide and carbendazim as a fungicide; all are commercially available readily for various agricultural and domestic purposes. The hypothesis tested in this investigation is that pesticide exposure during pregnancy causes changes in biomarker responses like serum glucose level, total protein, total cholesterol, triglycerides, SGOT, SGPT, and billirubin level. Significant changes were observed in all above biomarker responses, when compared with the reference. Histopathology of skin and kidney of rat neonates showed marked damage. Degenerative changes and vacuolization with eroded capsule were observed in kidney sample and thinning of epidermis in skin sample was seen in pesticides exposed neonates of rats. The serum biochemistry and histopathological findings are valuable markers for observing the changes caused by pesticide exposure.

本实验旨在探究暴露于草甘膦（glyphosate）与多菌灵（carbendazim）两种农药对怀孕雌性大鼠健康的影响。草甘膦作为除草剂使用，多菌灵则为杀菌剂，二者均易于商业获取，可应用于各类农业及民用场景。本研究检验的假说是：妊娠期间暴露于农药会引发血清葡萄糖水平、总蛋白、总胆固醇、甘油三酯、谷草转氨酶（SGOT）、谷丙转氨酶（SGPT）及胆红素水平等生物标志物响应的改变。与对照组相比，上述所有生物标志物响应均出现显著变化。对大鼠新生幼崽的皮肤与肾脏组织开展组织病理学检查后发现，其存在明显损伤：肾脏样本可见退行性改变、空泡变性及包膜糜烂，农药暴露组的大鼠新生幼崽皮肤样本则出现表皮变薄现象。血清生化指标与组织病理学检查结果，可作为监测农药暴露所致机体变化的有效标志物。