Evaluation of the reconstruction accuracy of tomoseq using simulated tomo-seq data.

For each simulating setting and spatial gene, 10 simulated tomo-seq data were generated. tomoseqr was applied to these data with the inter-section normalization option. Means and standard deviations of PCCs of the reconstruction results with the ground truth across 10 simulated tomo-seq data are shown. P-values were calculated by randomly shuffling the reconstructed results and calculating PCCs of the randomized results with the ground truth 1,000 times, following Kolmogorov-Smirnov tests (Materials and methods).