Metabarcoding analysis for detecting rapid onset of cultivation bias in marine sediment bacterial communities

For time-series metabarcoding analysis, we do sequencing 16S rRNA gene Amplicon Sequence for bacteria in marine sediments. We select for 5 time points, 0 h(pre-cultivation), 6h, 12h, 18h, 24h. At each time points, we extracted DNA from collected samples for further analysis.