Intracellular Ca2+ release by mononuclear (MN) colostrum phagocytes from diabetic mothers indicated by fluorescence intensity.

Phagocytes were pre-treated or not with 8-(Diethylamino) octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8) and incubated with melatonin (MLT), N-acetyl-serotonin (NAS), luzindole or chloromelatonin (CMLT) and loaded with the fluorescent radiometric calcium indicator FLUO3-AM (Fluo3-Acetoxymethyl) as described in Materials and Methods. Results are expressed as mean and SD (N = 5 per treatment).*indicates difference from the FLUO3-AM (ANOVA, P+indicates intergroup differences within each treatment (ANOVA, P#indicates differences between TMB-8 use within each treatment and group.