Glycosylation-related gene expression in the mucous-secreting gastrointestinal cell line HT29-MTX-E12 in response to infection by Campylobacter jejuni

The HT29 derivative cell line HT29-MTX-E12 (E12) produces an adherent mucus layer predominantly of the gastric MUC5AC mucin when grown on transwells. E12 cells were infected with C. jejuni and the transcriptome of infected and uninfected E12 were compared. The study focused on glycosylation related genes as these regulate the structures that have a major role to play in pathogen-host cell interaction. This experiment is related to another experiment where the cell line (E12) is replaced by the parental cell line (HT29) and importantly HT29 cells do not produce an adherent mucus layer. Three control uninfected E12 replicates were compared to two C. jejuni (11168) infected E12 replicates