Effect of P2 with other pro-oxidant molecules on macrophage viability and membrane integrity.

Macrophage are exposed to P2 (90 µg) alone or in combination with other pro-oxidant molecules present in malaria culture supernatant for 6 hrs and viability was determined by MTT assay where as membrane integrity was measured by LDH release assay. Macrophage exposed to hemin (60 µg/ml), heme polymer (40 µg/ml) was used as control. Change in cellular viability after reconstitution was calculated considering viability of P2 exposed macrophage as 100%. NA = “Not applicable”.