Transcriptomic analysis of pea plant responses to chitooligosaccharides' treatment revealed stimulation of calcium-regulated reactions and mitogen-activated protein kinase cascade

Since chitooligosaccharides are water-soluble, biodegradable and nontoxic compounds, their application may be considered as a promising plant-protecting agent. However, the molecular and cellular modes of action of COs are not yet understood. Transcriptional changes in pea roots treated with COs were investigated via RNA sequencing. Pea roots treated with the deacetylated CO8-DA at low concentration (10-5 ?) were harvested 24 h after treatment and their expression profiles were compared against medium-treated control plants. We observed 886 differentially expressed genes (fold change = 1; p-value ? 0.05) 24 h after treatment with CO8-DA. Overall design: Comparative gene expression profiling analysis of RNA-seq data for CO8-DA-treated pea Pisum sativum L. plants with plants incubated in medium without elicitors.