Genomic analysis of lncRNA and mRNA profile in circulating exosomes of patients with rheumatic heart disease. Genomic analysis of lncRNA and mRNA profile in circulating exosomes of patients with rheumatic heart disease

Rheumatic heart disease (RHD) is still a serious life-threatening heart disease in developing countries. Accumulating evidence suggested that circulating exosomes and its cargoes including mRNA, microRNA and long noncoding RNA (lncRNA) play essential roles in cardiovascular diseases, while their roles in RHD remain unexplored. In the present study, we investigated genomic profiles of circulating exosomes harvested from RHD patients. And we performed gene ontology (GO) and pathway analysis to explore potential roles of differentially expressed mRNAs. Furthermore, we identified 5 lncRNAs and their flanking coding genes simultaneously dysregulated in the circulating exosome. Collectively, we provided the first transcriptome analysis identifying lncRNAs and mRNAs in circulating exosomes in RHD patients, and our data provide valuable insights into seeking for potential biomarkers and therapeutic targets for RHD. Overall design: Microarray expression profiles of mRNAs and lncRNAs in circulating exosomes extracted from 5 RHD patients and 5 normal people were conducted .