Rna-Seq data of control Luc-kd and K14-kd shRNA transduced tumor organoids. Mus musculus

Conventional models of cancer progression propose that single cells leave the primary tumor, enter the circulation, and seed clonal metastases. However, metastases can contain multiple clones, raising the question: how do polyclonal metastases form? We demonstrate that cancer cells seed distant organs as cohesive clusters, composed of two molecularly distinct subpopulations, whose proportions vary systematically during metastasis. We establish that collective dissemination is a frequent mechanism for metastasis and identify a molecular program in the most invasive, keratin 14+ (K14+) cancer cells, regulating cell-cell adhesion, cell-matrix adhesion, and immune evasion. We demonstrate that this metastatic phenotype is dependent on K14 expression. Understanding the molecular basis of collective dissemination may therefore enable novel prognostics and therapies to improve patient outcomes. This Bioproject includes our RNA-seq experiments of MMTV-PyMT tumor organoids stably transduced with control Luciferase-knockdown shRNA or K14-knockdown shRNA.