Mechanomics RT-DC Set 06 - Developing Neurons

Entry is a part of Mechanomics collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:Urbanska, M., Ge, Y. et al.,De novo identification of universal cell mechanics gene signaturesBiorXiv: https://doi.org/10.1101/2021.04.26.441418; first published: 27th April 2021, last updated: 4th Nov 2024eLife: https://doi.org/10.7554/eLife.87930; first published: 23rd March 2023, last updated: 27th Dec 2024This dataset contains mechanical measurement of three populations of developing neurons isolated from the lateral cortices of double reporter E14.5 mouse embryos (Btg2RFP/Tubb3GFP):• proliferating progenitors (PPs) - RFP–/GFP–• differentatiating progenitors (DPs) - RFP+/GFP–• newborn neurons (NNs) - GFP+.Cells were isolated according to previously established procedures (Aprea et al., 2013, EMBO J).Measurements were performed using real-time deformability cytometry (RT-DC)._rtdc.zip - contains data acquired during the measurments in '*.rtdc' format_tsv.zip - contains extended data in a '*.tsv' format_glmm.zip - contains result summaries of statistical analysis comparing Young's moduli of the different cell types using generalised linear mixed effects modelsAprea, J. et al. Transcriptome sequencing during mouse brain development identifies long non-coding RNAs functionally involved in neurogenic commitment. EMBO J. 32, 3145–3160 (2013) https://doi.org/10.1038/emboj.2013.245