Transcription profiling of mouse ovaries from Lhx8 Null newborns

Lhx8 is a homeobox gene expressed in oocytes and critical in oogenesis. Lhx8 deficiency leads to rapid loss of postnatal oocytes. Early oocyte differentiation is poorly understood. We hypothesized that lack of Lhx8 perturbs global expression of genes preferentially expressed in oocytes. We compared Lhx8 knockout and wild type ovaries using Affymetrix 430 2.0 microarray platform. Experiment Overall Design: Each array was hybridized with cRNA produced from pooled total RNA from at least 25 specimens. The experiment was performed with 3 replicates. The results from these three arrays was compared to arrays hybridized with cRNA produced from pooled total RNA from at least 25 wild type specimens of the same genetic background.Lhx8是一种在卵母细胞中表达的同源框基因（homeobox gene），在卵子发生过程中发挥关键调控作用。Lhx8缺陷可导致出生后卵母细胞快速丢失。当前学界对早期卵母细胞分化的分子机制尚缺乏深入认知。我们提出假说：Lhx8的缺失会扰动卵母细胞中优先表达基因的全局表达谱。本研究采用Affymetrix 430 2.0微阵列（Affymetrix 430 2.0 microarray）平台，对Lhx8敲除型与野生型卵巢组织进行了比较分析。实验整体设计：每张微阵列均与来自至少25份混合标本的总RNA制备的cRNA进行杂交。本实验设置3次生物学重复。将上述3组敲除型阵列的杂交结果，与来自相同遗传背景下至少25份野生型标本的混合总RNA制备的cRNA杂交所得的阵列结果进行对比分析。