Comparison of RR-P autodephosphorylation rates with the RR-P dephosphorylation half-times predicted by the model.

*These values were calculated from the experimentally determined in vitro autodephosphorylation rate constants [48].
†The model was allowed to reach a steady state where CheA2 autophosphorylation (reaction 1) and the phosphorylation of CheA3 by CheA4 (reaction 2) were both active. Then reactions 1 and 2 were turned off. These half-times represent the time taken for levels of each of the RR-Ps to fall to half of their steady state values. Deletion of RRs was simulated by setting their total concentration in the model to zero, e.g. for ΔcheY3, Y3T = 0 (Table 2).
‡Lack of CheA3 phosphatase activity was simulated by setting k15a = k15b = 0 (Table 2).