Human Rhinovirus Infection of the Respiratory Tract Affects Sphingolipid Synthesis

We report the application of bulk RNA sequencing to RNA extracted from lung tissue and magnetic bead isolated EpCAM+ lung epithelial cells and CD45+ lung immune cells from mice. Heterozygous serine palmitory transferase subunit 2 , Sptlc2+/- (SPT) and Sptlc2+/+ controls (WT) mice were included. Differential gene expression analysis reveals genes within the sphingolipid synthesis pathway are similarly expressed in the lung and lung epithelial cells of RV infected WT mice and SPT mice (both infected and uninfected). Overall design: mRNA profiles from lung tissue, lung EpCAM+ epithelial cells and lung CD45+ immune cells collected from 8 week old WT and SPT mice, 24 hours after after intrasal innoculation with human rhinovirus (HRV)or PBS