Developmental exposure to the Fox River PCB mixture modulates behavior in juvenile mice

Developmental exposures to PCBs are implicated in the etiology of neurodevelopmental disorders (NDDs). This observation is concerning given the continued presence of PCBs in the human environment and the increasing incidence of NDDs. Previous studies reported that developmental exposure to legacy commercial PCB mixtures (Aroclors) or single PCB congeners found in Aroclors caused NDD-relevant behavioral phenotypes in animal models. However, the PCB congener profile in contemporary human samples is dissimilar to that of the legacy Aroclors, raising the question of whether human-relevant PCB mixtures similarly interfere with normal brain development. To address this question, we assessed the developmental neurotoxicity of the Fox River Mixture (FRM), which was designed to mimic the congener profile identified in fish from the PCB-contaminated Fox River that constitute a primary protein source in the diet of surrounding communities. Adult female C57BL/6 J mouse dams (8-10 weeks old) were ex..., Fox River PCB Mixture
The Fox River PCB Mixture (FRM) is a synthetic PCB mixture containing 35% Aroclor 1242, 35% Aroclor 1248, 15% Aroclor 1254, and 15% Aroclor 1260 by weight (22). All Aroclors for this study, including Aroclor 1242 (catalog #: C-242N, lot #: 0114-A), Aroclor 1248 (catalog #: C-248N, lot #: 1063-24B), Aroclor 1254 (catalog #: C-254N, lot #: 124-191-B) and Aroclor 1260 (catalog #: C-260N, lot #: 021-020-1A) were purchased from AccuStandard, Inc. (New Haven, CT, USA). The FRM was prepared by adding 525 mg of Arclor1242, 525 mg of Aroclor 1248, and 225 mg of Aroclor 1254 to a vial containing 225 mg of Aroclor 1260. The mixture was kept at 30 °C for 5 min and homogenized by shaking. 
The FRM was dissolved in peanut oil at a stock concentration of 20 mg/ml and stored in amber glass vials at -20 °C. A 12.5 µL or 125 µL aliquot of 20 mg/ml peanut oil/FRM stock was mixed into 10 grams of peanut butter with a metal spatula for 5 min daily before use.  Organic peanut butter (Tr..., , # Developmental exposure to the Fox River PCB mixture modulates behavior in juvenile mice

[https://doi.org/10.5061/dryad.2jm63xt0c](https://doi.org/10.5061/dryad.2jm63xt0c)

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Developmental exposures to PCBs are implicated in the etiology of neurodevelopmental disorders (NDDs). This observation is concerning given the continued presence of PCBs in the human environment and the increasing incidence of NDDs. Previous studies reported that developmental exposure to legacy commercial PCB mixtures (Aroclors) or single PCB congeners found in Aroclors caused NDD-relevant behavioral phenotypes in animal models. However, the PCB congener profile in contemporary human samples is dissimilar to that of the legacy Aroclors, raising the question of whether human-relevant PCB mixtures similarly interfere with normal brain development. To address this question, we assessed the developmental neurotoxicity of the Fox River Mixture (FRM), which was designe...